Black sesame seeds are considered as a beneficial medicine. In this work, sesame seeds were extracted using honey as solvent. The effect of ratios (w/v) of sesame and honey on the total phenolic content and antioxidant activity of sesame seed extract were evaluated to determine the most suitable ratio of extraction. Total phenolic content and antioxidant activity of extract were measured according to the Folin–Ciocalteu methods and DPPH methods using gallic acid and 3,5-di-tert-4-butylhydroxytoluene (BHT) as standard, respectively. The results revealed that the highest amount of total phenolic content and activity in scavenging DPPH free radicals were observed in the ratio of 1:1 w/v of sesame and honey with the amount of 4.22%±0.03 mg gallic acid equivalent/g extract and 62.05%±0.38 (BHT at 57.08%±0.46), respectively. This extract was investigated the nutritional information. It composes of sesamin 55 mg/100 g extract, omega3, omega 6, omega 9, boron, 10 essential amino acids and other nutrients. Moreover, absorption of sesamin in rats was investigated from their serum after a single oral dose of extract 3.2 g/kg in rats using HPLC. The results indicated that sesamin can be absorbed at first for 15 minutes and exhibited the highest sesamin at 2.0 h.

ในการประชุมวิชาการระดับนานาชาติ 13-14 กุมภาพันธ์ 2020 Paccon2020 นำเสนอในหัวข้อ

Bioactivities of Black Sesame Seeds Extract Prepared using Non Chemical Extraction Processes and Absorption of Sesamin in Rats

Black sesame seeds are considered as a beneficial medicine. In this work, sesame seeds were extracted using honey as solvent. The effect of ratios (w/v) of sesame and honey on the total phenolic content and antioxidant activity of sesame seed extract were evaluated to determine the most suitable ratio of extraction. Total phenolic content and antioxidant activity of extract were measured according to the Folin–Ciocalteu methods and DPPH methods using gallic acid and 3,5-di-tert-4-butylhydroxytoluene (BHT) as standard, respectively. The results revealed that the highest amount of total phenolic content and activity in scavenging DPPH free radicals were observed in the ratio of 1:1 w/v of sesame and honey with the amount of 4.22%±0.03 mg gallic acid equivalent/g extract and 62.05%±0.38 (BHT at 57.08%±0.46), respectively. This extract was investigated the nutritional information. It composes of sesamin 55 mg/100 g extract, omega3, omega 6, omega 9, boron, 10 essential amino acids and other nutrients. Moreover, absorption of sesamin in rats was investigated from their serum after a single oral dose of extract 3.2 g/kg in rats using HPLC. The results indicated that sesamin can be absorbed at first for 15 minutes and exhibited the highest sesamin at 2.0 h.

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