Research project on Integrated Waste Management and Utilization of Small Pig Farm was firstly planned for 2 years and comprised of 4 subprojects. There are 1) Design and Development of Prototype of Mixed Anaerobic Digester for Biogas Production from Small Pig Farm, 2), Isolation of sulfur oxidizing bacteria for hydrogen sulfide removal, 3) Bio-Hydrogen Production from Swine Manure Using Anaerobic Fermentation and 4) The Study on the Efficient Testing of Soil Amendment and Fermented Liquid Fertilizer Production from Sludge and Effluent from Prototype of Mixed Anaerobic Digester for Vegetable Production. All subprojects showed progressive results and hopefully, beneficial enough for the project next year
The objective of this research is to study the effect of mixing on anaerobic digestion, biogas production and swine wastewater treatment in pilot scale. Four sets of experiments were performed using swine wastewater feed and gas began to fill up of water a day in the different mixing 2, 4 and 8 times a day (30 min/times ) and (control) unmix were performed in 1000 liters bioreactor .Bioreactor ware started up by swine wastewater, and operated biogas produced. After that, swine wastewater was feed to all reactors 100 liters per day throughout experiment. It was found that efficiency that the mixing had affected to biogas production and swine wastewater treatment efficiency.
Soil samples were collected from natural farms and factories. Include sewage sludge sample of 30 sources. When taken into isolation. Sulfur-Oxidizing Bacteria on culture medium, Thiosulfate Agar and Thiosulfate Broth at pH 4 and 7 were purified, stored total 51 isolates; only 19 isolates were able to grow in wastewater from pig farms. Found that the bacteria were not able to grow at pH 3 only, and were highly effective in the removal of sulfide at pH starting at 8 when bacteria WI023, WI048 and SLP02 to classification by sequencing of 16S. rRNA showed that the bacteria Thiobacillus sp., Thiobacillus ferrooxidans and Acinetobacter sp. respectively.
Biohydrogen production from swine farm wastewater by two-phase anaerobic fermentation was investigated in this research. The inoculum sludge was heat-treated at 100 OC for 30 minutes. The two-phase bioreactors for hydrogen production consisting of acid tank and hydrogen production tank with 60 L in total volume and 36 L in working volume were design. The hydraulic retention times (HRT) of 12, 24, 36, and 48 h under controlled pH at 5.0 were studied. The maximum hydrogen concentration of 540 mg/m3 was occurred at HRT of 24 h.
This objective of experiment was to study the efficiency of soil amendment and liquid fertilizer production from sludge and effluent from prototype of mixed anaerobic digester. In this experiment, tests with Swine waste of Maejo University’s animal science and technology, with the agitation rate to 8 times per day by the water effluent to increase the efficiency. The germination test of digester sludge and effluent water were found the germination index similar to the standard of organic fertilizer and the digester sludge also increases the organic matter in the soil.