This study employed a quasi-experimental, two-group post-test design to investigate the impact of Clinical Nursing Practice Guideline for premature newborn receiving non-invasive ventilator support on complications associated with this form of respiratory support. A sample of 60 participants was selected based on specific criteria and divided into two groups: a control group (n=30) receiving standard nursing care and an experimental group (n=30) receiving care according to the Nursing Guidelines for neonatal respiratory support. Research instruments included predetermined neonatal nursing practices for infants receiving non-invasive respiratory support, a general data recording form, and an assessment form for pressure ulcers around the nose, nasal obstruction, and abdominal distension. Statistical analysis involved comparing general data between the control and experimental groups using the Mann-Whitney U-test and independent t-test, and comparing the incidence of complications using the chi-square test. The study found no statistically significant differences in general data between the groups (p >.05). However, the experimental group exhibited a significantly lower incidence of pressure ulcers around the nose, nasal edema, and abdominal distension compared to the control group (p< .05). These findings suggest that adhering to predetermined neonatal nursing practices for infants receiving non-invasive respiratory support may reduce complications associated with this form of respiratory support.