The high quality of freshwater macroalgae; Spirogyra neglecta is a high demand for local consumption found in Northern Thailand. Productivity increasing capacity of high-quality Spirogyra algae with a lift tray in closed water recirculation system using precision farming technology is a 3-year project. The objectives of the first year were 1) to find the optimum level of nutrient which discharge from fish pond for Spirogyra growth in movable-plastic ponds, 2) to compare the contents of bioactive compounds between 2 culture types; movable-plastic ponds and earthen ponds in a private farm, and 3) to improve quality of Spirogyra algae using ozone micro-bubble technique. The results showed that the most effective nutrient content for Spirogyra algae production was at 20 ?s/ cm (p <0.05) of water conductivity. For comparative farm study, the higher levels of antioxidant levels, polyphenols contents were found in Spirogyra algae which from movable-plastic ponds production system. In addition, soaking Spirogyra algae in water with small air-ozone bubbles (Ozone micro-bubble) could improve the quality of algae and could increase both the antioxidants and polyphenols content (p <0.05).