The study on the effects of animal manure on growth, yields and flavonoids content of Kaempferia parviflora Wallich. ex Baker. was carried out by applied 500 or 800 kg/rai of five different animal manures (cow manure, chicken manure, swine manure, guano and vermicompost). The experiments were conducted between March 2019 and March 2020 at the demonstration farm of Maejo University, Chiang Mai. The highest nitrogen solubility was found in the chicken manure, highest phosphorus in the swine manure and highest potassium in the vermicompost. The results shown that no significant of pseudostem size, leaf size, number of shoots and flavonoids content on the different of application rates and types of the manure. However, the height application rates of all animal manure types shown height weight of rhizome more than low application rates that harvested at 32 weeks.