Curcuma alismatifolia is an important cut flower in Thailand which cannot be produced all year-round due to the environmental factors. Meeting the market requirements is necessary for off-season flower production. Since flowering time control in Curcuma in Thailand is done using the primitive methods such as photoperiod and temperature control. Hence, challenges of improving the method of production or the plant variety will lead to the increasing of their value which can be exported to global market. Therefore, the study of flowering genes in Curcuma is needed in order to develop the new varieties which can be produced all year round. There are two parts of this project, the study of flowering time physiology using transfer experiment and the study of flowering time gene expression using transcriptome sequencing. The results from the physiology study show that Curcuma is a facultative long day plant which flower can be produced quicker under LD condition. Moreover, RNA-Seq data shows at least 122 differentially expressed genes involved in flowering which related to carbohydrate metabolism, flowering time, growth and development, and plant defense mechanism. Key genes can be selected base on the function for further study to improve new Curcuma variety which can be produced off-season.