Production of economic aquatic animal in prototype community for food safety to Clarias gariepinu and environment, the four sub-projects had integrated as follows: Project 1 : The research aimed to compare growth, nutrition values, pigments and water quality. There were two experimental trials: Experiment 1: the cultures of Aspi.MJU2 in the catfish pond water (CPW), the concentrations of CPW ranged 10%-100% at the laboratory. It can be concluded that the cultivation of Aspi.MJU2 in the 80%CPW had better growth (Cell count) of Aspi.MJU2 than other treatments. Experiment 2: The CRD was applied, there were four treatments, with 3 replications, Algae were cultured in the closed glass cabinets: T1 Aspi.MJU2 cultured in MZm, T2 Aspi.MJU2 cultured in the catfish pond water (80%CPW+NaCl+N:P:K), T3 Aspi. MJU2 cultured in 80%CPW+NaCl and T4 Aspi. MJU2 cultured in 80%CPW+N:P:K. The data were collected every 5 days for a 15-day culture period. The result showed that: MZm and 80%CPW+NaCl+N:P:K provided greater growth rate of specific cells, production, protein and carotenoid better than other treatments. But 80%CPW+NaCl+N:P:K had better production costs other treatments.
Project 2 : Experiments were carried out to investigate the suitability of using chicken intestine as raw material for silage production and the effect of silage blended with rice bran or soybean meal in the diet of African catfish (Clarias gariepinus). In the first stage, to evaluate the efficiency of different concentration of citric acid to fermented chicken intestine at room temperature for 30 days. Acid silages were prepared using chicken intestine supplemented with four different concentrations (4% 6% 8% and 10%) of citric acid. After 30 days of fermentation, results indicated that protein content in silage fermented with citric acid 8% and 10% were significantly (p<0.05) higher than those fermented with 4% and 6%. In the next stage, to produce feed silage 10% 20% 30% 40% and 50% rice brand (RB10, RB20, RB30, RB40 and RB50 respectively) and 20% 30% 40% and 50% soybean meal (SB20, SB30, SB40 and SB50 respectively) were mixed in liquid silage. The results showed that protein and lipid content in feed silage significantly (p<0.05) decreased with percentage of rice brand. However, protein content in feed silage significantly (p<0.05) increased with percentage of soybean meal and vice versa, on lipid content. The last trial, nine experimental diets containing various levels of rice bran (RB10, RB20, RB30, RB40 and RB50) or soybean meal (SB20, SB30, SB40 and SB50) from second trial and a commercial catfish feed as a control diet (Control) were fed triplicate groups of 30 fish (initial weight: 16.05 g) at 5% body weight per day for 60 days. A significant higher (p<0.05) in weight gain, specific growth rate (SGR) and lower feed conversionratio (FCR) was observed in fish fed on Contr diet compared to those fed rice bran inclusion diets (RB10, RB20, RB30 and RB40) and some soybean meal inclusion diets (SB30, SB40 and SB50). However, the growth performance was similar in fish fed Contr and SB20 (p>0.05). No significant differences were detected in survival among fish fed any dietary treatments (p>0.05). The carcass protein content was similar in all treatments (p>0.05). Lipid content in fish fed soybean meal inclusion diets (SB20, SB30, SB40 and SB50) was significant lower (p<0.05). The study demonstrated that up to 20% of soybean meal could be incorporated in silage diets of Clarias gariepinus without negative effects on growth and carcass composition.
Project 3: Diversity identification of aquatic worms found in Thailand from 2 sources including pet shop in the central region and natural ditch at Sansai district, Chiang Mai province was conducted by external morphological features concluded that there are different aquatic worm species per region. Aquatic worms from central region had 4 Families 5 Genus such as 38.64% Tubifex sp. (Tubificidae), 30.85% Nais sp. (Naididae), 22.50% Aeolosoma sp. (Aeolosomatidae), 4.86% Branchiura sowerbyi (Tubificidae) and 3.10% Haplotaxis sp. (Haplotaxidae) respectively. Aquatic worms from Chiang Mai province had 3 Families 3 Genus such as 85.81% Aeolosoma sp. (Aeolosomatidae), 11.23% Branchiura sowerbyi (Tubificidae) and 2.96% Nais sp. (Naididae) respectively.
A study was conducted on a proper diet to feed aquatic worms (4 treatments, each treatments had 3 replications) using minced fish, decayed vegetable, yeasts and jellies for 4 weeks. It was concluded that aquatic worms fed with yeast had the best growth even through decline growth curve but no different fed with decayed vegetables (p>0.05). Aquatic worms fed with decayed vegetables and yeast had a higher growth rate than those fed with minced fish and jellied (p<0.05). Weight checking at aquatic worm 2 times (before and after demonstration), it was found that aquatic worm fed with yeast had the best growth and an increased growth curve but no different fed with decayed vegetables (p>0.05) and growth higher than jellied (p<0.05)
The study was conducted on the possibility of using aquatic worms to dispose of waste at the bottom of fish pond under aquaculture system with sustainable and environmentally friendly condition from culture catfish using 4 different quantities of aquatic worms (4 treatments 3 replications) for 12 weeks. It was concluded that compared with using only culture catfish (p<0.05) the additional use of aquatic worms to dispose of waste at the bottom of the pond because decreased organic matter in the soil and deceased ammonia in the water.Project 4 : Product development of fish finger and fish burger from African catfish (Clarias gariepinus). The purpose of this research was to study the effect of washing of fish mince on quality changes during storage of frozen fish finger and fish burger at -18 ? C for 5 months. The samples were determined for quality changes at 0, 1, 2, 3, 4 and 5 months. It was found that the chemical composition, i.e., moisture, fat, protein and ash contents, of washed mince decreased significantly (p <0.05). The pH and TBAR of the washed mince were decreased, while the amount of soluble protein or myofibrillar protein was increased (p <0.05). The fish finger products have good nutrition as follows:- the moisture, fat, protein and ash contents were in the ranges of 40.84-43.88, 32.45-35.67, 12.84-13.53, and 1.42-1.74 %, respectively as the storage time (at -18 ? C) increased. The soluble protein contents is likely to decrease and the TBARS value is likely to increase. Therefore, mince washing could improve keeping quality of frozen fish finger.