Fresh butterfly pea was extracted with 95% ethanol and analyzed the antioxidant activity
using DPPH scavenging activity and total phenolic content. The total phenolic content of butterfly
pea ethanolic extract (BE) was 26.90?1.12 mg gallic acid equivalents/g of extract and radical DPPH
scavenging activity of ethanolic extract was 27.78?0.12 mg Trolox equivalents/g of extract. The
ethanolic extract-loaded Nanostructured Lipid Carriers called BE-NLC was prepared by the high
shear homogenization method using compritol 888 ATO, miglyol oil, poloxamer 188, extract and
tween 80 and then determined the particle size, polydispersity index, zeta potential and entrapment
efficiency. The results showed that the morphology of the BE-NLC was spherical. The entrapment
efficiency of extract was 72.51?1.11% in NLC system. Antioxidant potential of BE and BE-NLC for
stabilization of fish oil was evaluated and compared with butylated hydroxy toluene (BHT) as
synthetic antioxidant. This antioxidant potential was evaluated by the estimation of peroxide values
(PVs) of catfish oil by treated with natural antioxidants (BE and BE-NLC) and BHT by incubating at
65?C for a period of 12 days. The results revealed that BHT had higher antioxidant activity than BE
and BE-NLC in fish oil. In addition, treatment containing BE-NLC and BHT in fish oil performed
similar PVs after the 4 days. However, the antioxidant activity of BE-NLC was higher than that of
BE in stabilization of fish oil.