Commercial dielectric barrier discharge plasma jet (DBDPJ) with helium gas was used as a handy cold atmospheric pressure plasma tool to study the DBDPJ
effects on contaminated wound healing. The contaminated wound was modeled into three parts: bacteria as the contaminant origin, bacterial biofilm as the
simulated bacterial contaminant layer on the wound, and the wound cells. The bacteria were Staphylococcus aureus and Pseudomonas aeruginosa and the
wound cells were human dermal fibroblasts adult (HDFa) cell. DBDPJ treatments were carried out on the three model parts respectively to study the DBDPJ
effects on bactericidal and wound cell survival as functions of the plasma conditions including the plasma dissipated power and exposure time and the role
played by the plasma radical species. The results demonstrated that DBDPJ could effectively kill the bacteria without significant side effect on the HDFa cell.
Optimal plasma conditions were determined and are ready for clinical uses.