This study was aimed to extract the bioactive compounds from black glutinous rice (Oryza
sativa L.). The three varieties of black glutinous (Leum Pua, Doi Saket, and Phayao) were extracted
using distilled water, citric acid solvent and ethanolic citric acid solvent. The influences of
temperature, time and pH value on total phenolic content and antioxidant activity (percentage of
inhibition of DPPH free radical) were investigated. The selected solvent was citric acid solvent with
the selected optimum conditions were 72.0 ?C, 91.1 mins at pH 1.44 for Leum Pua, 72.5?C, 83.0
mins at pH 1.39 for Doi Saket, and 66.3?C, 74.7 mins at pH 1.32 for Phayao. The effect of cold
oxygen plasma treatment at 10, 20 and 30 min on cyanidin-3-O-glucoside content was studied. The
result found that plasma treatment offered superior cyanidin-3-O-glucoside content compared with
untreated plasma sample. The optimum condition was 20 min. The cyanidin-3-O-glucoside content
of Leum Pua, Doi Saket, and Phayao extracts were increased from 202.04 to 382.64 ppm, 10.19 to
19.46 ppm, and 10.97 to 18.62 ppm, respectively. The extracts were dried using spray drier and
were then investigated in in vitro digestion process. The results showed that cyanidin-3-O-glucoside
content slightly decreased after gastric digestion process, therefore, this bioactive compound can
maintain its property for benefit in intestinal digestion process. The application of bioactive
compound of low temperature plasma treated black glutinous rice for probiotic growth promoter
was then evaluated and the result demonstrated the probiotic growth enhanced due to adding of
colorant powder prepared from glutinous rice extract. This study concluded that the bioactive
compound of low temperature plasma treated black glutinous rice can be used as probiotic growth
promoter and colorant in yogurt.