This study aimed to examine the appropriate organic carbon sources to produce biofloc concentration for climbing perch and mad carp, growth performances and the appropriate aeration position for mixed the water through culture period. The individual molasses, molasses combined with wheat flour and molasses combined with rice bran in 50 : 50 ratio were designed for three organic carbon sources. The carbon and nitrogen ratio (C : N ratio) was control with 15 ratio in value through culture period. The stocking density of climbing perch and mad carp was defined with 40 and 30 fishes per m2. Theirs were fed under climbing perch and mad carp feeding program respectively. The experiments were conducted within 6 months respectively. The water quality particularly dissolved oxygen (DO) was maintained at least 5 ppm and when the ammonia was reached 1 ppm external carbon sources were prepared and computed and diluted in the water immediately. The results showed there were non-significance difference within weight gain, average daily weight gain, feed conversion ratio, specific growth and survival rate among three carbon sources (P>0.05). Moreover, there was non significance on the concentration of biofloc from difference carbon sources. Finally, this result showed that the combination of molasses and rice bran, wheat flour could be induce bioflioc in many fish species culture.