Objective: This descriptive study aimed to examine satisfaction of nursing lecturers using rubber manikins for venipuncture and peripheral intravenous cannulation practice. Methods: Purposive sampling was used to recruit 102 nursing lecturers in 10 networked nursing education institutions teaching basic nursing skills or subjects related to the use of manikins for venipuncture and peripheral intravenous cannulation practice between December 2023 and February 2024. The questionnaires used in the study included: 1) the general information questionnaire; and; 2) satisfaction questionnaire about the use of simulation manikins for venipuncture and peripheral intravenous cannulation practice. Content validity for the scale was .82 and Cronbach’s alpha coefficient was used to examine the reliability of the satisfaction questionnaire, yielding a value of 0.81. The data were analyzed using descriptive statistics. Results: The research result show that the overall satisfaction of nursing instructors is the highest level (M = 4.26, SD = .56). The top three aspects of satisfaction with efficiency include: safety in usage (M = 4.56, SD = 0.57), the manikins meeting operational needs (M = 4.47, SD = .75), and ease of use without complexity (M = 4.46, SD = .68). The top three aspects of satisfaction with the design include the creativity in the manikins’ creation (M = 4.52, SD = .67), the appropriateness of shape and color (M = 4.36, SD = .71), and the neatness and aesthetic of the manikins (M = 4.35, SD = .70). Conclusion: The development of educational tool for vascular access using natural rubber has achieved the highest level of satisfaction, with properties that closely simulate human skin. This allows for an effective replacement of expensive simulators and also enhances the value of natural rubber products.Keywords: satisfaction; nursing lecturer; venipuncture and peripheral intravenous cannulation manikins