Nowadays, a trend of rice with high nutritional value or with special properties has continuously increasing. Therefore, such rice varieties should be studied and selected,
to cultivate and increase productivity for exportation by employing marketing strategies in terms, special properties, and distinct packaging of public interest. Thus, this project aims to select, study, and yield test of non-photoperiod sensitive, semi dwarf, aromatic non-glutinous, resistant to diseases and insect, and high nutritional value of Hom Mali Dang rice lines. During the first year (2020), season 1 (rainy season 2019), an observation trial, planted 2 rows of 50 F7 lines
non-photoperiod sensitive, semi dwarf, aromatic non-glutinous, resistant to diseases and insect and high nutritional value of Hom Mali Dang and the 16 plots with the highest yield potential were selected. It was found that the between yield was 341-775 kg per rai. Then, 298 plants with good agricultural characteristics were selected. Self-pollinated F8 seeds were produced and selected by molecular markers; Bph3, xa5, qBL1, qBL11. The most There were 105 lines with homozygous genotypes of the Bph3, xa5, qBL1, qBL11 gene were selected with the flowering date average of 75% in between 86-111 days. In season 2 (dry season 2020), the advance trial planted 4 rows of
35 F8 lines non-photoperiod sensitive, semi dwarf, aromatic non-glutinous, resistant to diseases and insect, and high nutritional value of Hom Mali Dang. and the 16 plots with the highest yield potential were selected. It was found that the between yield was 748-1,082 kg per rai with the flowering date average of 75% in 89-131 days. And 186 plants One hundred eighty-six lines with good agricultural characteristics were selected. As a result, the 16 lines of non-photoperiod sensitive, semi dwarf, aromatic non-glutinous, resistant to diseases and high nutritional value of Hom Mali Dang with the highest yield potential were selected and self-pollinated F9 seeds were produced. The, F9 seeds were then selected by molecular markers; qBL1, qBL11, fgr, Wx/wx, Wxa/Wxb, SSIIa, Rc, OsB1. It was found that all genotypes of the 16 lines were homozygous at qBL1, qBL11, fgr, Waxy, SSIIa, Rc, OsB1 genes. Thus, qBL1 and qBL11 genes control rice to have resistance to blast disease. The fgr gene controls the fragrant rice while waxy genes control
non-glutinous/glutinous rice on which all 16 lines were all non-glutinous. SSIIa gene controls rice to have a low starch gelatinization temperature. The Rc and OsB1 genes control the rice to red and black, respectively. These studies of the genotyping were able to select rice that have properties of fragrant rice, low starch gelatinization temperature, red and black colors of rice with high nutritional value. Therefore, they can be planted and further tested on Intra-station trial.