This program contains 3 projects following (1) dopamine biosensor, (2) glucose sensor, and (3) uric sensor. The first project was the dopamine biosensors development using gold nanoparticles (AuNPs) at carbon dots (CDs) sheathed by the chitosan (CHIT) (AuNPs@CDs-CHIT) and the tyrosinase enzymes (Tyr) on the surface of glassy carbon electrode (GCE) (Tyr/AuNPs@CDs-CHIT/GCE). The stability test of the developed dopamine biosensor found more than 35 times usage. The developed dopamine biosensors had been tested with real blood samples without having sample preparation. It provided a percentage recovery in the range of 96.00 - 98.80 percent. The second project was the development of a glucose sensor on the glassy carbon electrode based on Ni particles and ordered mesoporous carbon. It was found that the synthesized nickel particles have rose-like structures. From the results of glucose analysis using electrochemical techniques, it accelerated the reaction of glucose analysis with the signal to background (S / B) is equal to 1.59. The last project was the development of a uric sensor based on Cu metal corporated with graphene oxide (GO). The electrochemical investigation showed that the sensor performed good selectivity and wide linear ranges for the uric acid detection at 0.4 to 820 ?M and the detection limits was down to 0.24 ?M (S/N = 3) for analytes.