Resistant starch is beneficial for comsumer’s health. This research aimed to increase resistant starch content in rice which has good cooking and eating quality, and develop molecular marker linking with resistant starch content in rice. RD 43 was the donor, whereas, Pathum Thani 1 and RD Maejo 2 were recipients. Molecular markers that were used in hybrid selection were from three starch biosynthesis pathway genes; SSIIIa, SBEIIb and Waxy. The results showed that F1, F2, BC1F1, BC1F2 and BC2F1 hybrids were able to produce both from Pathum Thani 1 x RD 43 and RD Maejo 2 x RD 43. However, RD 43 had low amount of resistant starch and therefore the three action plans for year 2563 were prepared.