Physiological and Metabolomics analysis were analysed in longan peel (Dimocarpus longan
Lour. Var. Daw) from Ban Phu Din, Mae Taeng District, Chiang Mai Province. This research compared
the physiological disorders of longan peel after applied Zn and B solution at concentration (w/w) 0%
(T1), 0.1% B (T2), 0.2% B (T3), 0.1% Zn (T4), 0.2% Zn (T5), 0.1% B + 0.1% Zn (T6) และ 0.2% B +
0.2% Zn (T7). Metabolites were extracted from leaves after either Zn or B was applied at 0, 8, 16, 24
and 28 weeks and from fruit peel at 1, 2 and 4 months after fruit set. The analysis of LC-MS in the
metabolomics of leaves was found that total ion chromatogram (TIC) in terminal phase was disturbed
by leaf binding agents and surfactants. Therefore, it is unable to interpret the LC-MS data set to facilitate
the search for the metabolites from leaves that related to the physiological disorders or used as
biomarker. Therefore, the study of metabolite biomarker was studied from longan peel between 0.2%
w/w zinc treated longan fruit (T6) and the control that shown physiological disorder fruit (T1) at 1, 2
and 4 months after fruiting. It found that 2 important substances (6-(Hydroxymethyl)-2,4(1H,3H)-
pteridinedione (intermediate in folate biosynthesis) and ?-aminoheptanoic acid (inducer on immunity
to resist infection) significantly increased (q <0.05) in T6 group at 1 month after fruiting. However,
there were 2 important substances, 6-methyl thiohexylhydroximoyl-glutathione and trihomomethionine
decrease during the same period. These two substances decreased as same as the reduction of vegetative
storage protein (vsp) which found in the fruit from plant treated with Zn+ B.