Rubber is an important crop in the Thailand. The object of this research is to study the yield of rubber with different fertilizer . Performed at Lamae district, Chumphon province from October 2016 to September 2017. Completely randomized design was employed to determine statistical analysis of yield among those fertilizer formula. Analysis of variance showed that latex per area statistically significant. Treatment 4 Mixed Fertilizer No.2 CF(20-8-20) : AmiMate = 50:100 kilogram highest latex average of 2243 liters per rai per year. But not different with treatment 5 Mixed Fertilizer No.3 CF(20-8-20 : AmiMate = 50:150 kilogram receive latex average of 2239 liters per rai per year. but different treatment 1 CF (20-8-20), treatment 3 Mixed Fertilizer No.1 CF(20-8-20) : AmiMate = 50:50 kilogram and treatment 2 AmiMate? 5-3-5 receive average of latex was 2028, 1982 and 1952 liters per rai per year, respectively.