This research aimed to know the way to propagate Dendrobrium genus organic plantlet under tissue culture system. The plant propagation was done by tissue culture technique and used organic fertilizer with other organic extract for plant growth. D. chrysotoxum and D. aphyllum were used for the research study. The effect of concentration of plant and fish extract organic fertilizer had done for basic research. The result showed that both extract organic fertilizer concentration 2 ml/l was suitable for plant growth and it was not different from VW. However this result was unsatisfied, therefor the experiments had done continuously for studying the effect of other organic extract (coconut water, banana pulp, potato extract, yeast extract and malt extract) for butter growth. The result showed that plant extract organic fertilizer 2 ml/l + malt extract 1 g/l + potato extract 100 ml/l and add with coconut water 150 ml/l + banana pulp 100 g/l enhanced better plant growth for both D. chrysotoxum and D. aphyllum and the growth was not different from VW. Whereas the effect of media which contained only organic matters on shoot induction was not good as VW media. On the other hand, the result of chemical and organic fertilizer treatments by spying to plantlets in greenhouse cultivation showed that the number of new shoot both D. chrysotoxum and D. aphyllum were not different in all treatments. This result indicated that it might be the time of treatments was short for growth observation because normally the growth of these plants was slow in the nature.