Agricultuaral maize wastes are lignocellulosic materials that can be used for the ethanol production. In the ethanol production, the lignocellulosic materials were treated by using physical, chemical and enzymatic pretreatment. In this research, the agricultural maize waste were treated at ratio of dried plant and pretreatment solution at 1:5. The mixture solutions were heated at temperature 121?C, 1l.5 lb/in2 for 15 min. The acid pretreatment using the hydrochloric acid at concentration at 2, 3 and 4% (v/v) showed that the HCl concentration at 4% (v/v) release the highest content of cellulose after pretreatment. Then, the treated samples were treated again under the alkaline condition by using 2, 3, 4 and 15% (w/v) of sodium hydroxide solution. The results were suggested that at 15% (w/v) of NaOH showed the highest concetent of cellulose about 76%. After that the treated solids were digested by using cellulase under tempeture at 42?C and 200 rpm, the digestion times for cellulose degradations were about 27-36 hr that released the highest sugar concentration and the (%) saccharifications were nearly 100%. Then, the ethanol fermentations were done by using the sugar solutions from the steps of pretreatments under various initial sugar concentrations. The ethanol fermentation by Saccharamyces cerevisiae under temperature at 37 ?C for 72 hr showed that the both of ethanol yields and (%) thereotical ethanol yields were not significantly different and they were about 95 – 97% of thereotical ethanol yields.