Waterlogging in rice fields reduced weeds problem but the adverse effect of anaerobic condition on the growth of anaerobic bacteria and produced many fermentation products such as methane, carbondioxide and other substance. The accumulation of fermentation products in rice fields released to the environment had effect on growth and productivity of rice and also causes climate change. Under stressful condition, plants will accumulate large amounts of ethylene that effect on growth and development of plants and ethylene also causes aging in plants. ACC deaminase producing bacteria could reduce the production of ethylene, thereby reducing stress in plants. In addition, siderophore producing bacteria help the plant to control plant pathogen. In this research, screened ACC deaminase producing bacteria in tryptic soy broth (TSB). It was found that S70, PVKRL2, S143, S149, RLPVK1 and S216 could produce ACC deaminase at 0.030, 0.022, 0.016, 0.012. , 0.012 and 0.011 Unit/ml, respectively. The correlation of time on growth and ACC deaminase production of bacteria S70 showed the maximum ACC deaminase production 0.040 Unit/ml at 48 h. Bacterial S70 was Gram positive bacteria. The sequencing of the 16S rRNA gene of bacterial S70 was examined and found that it belonged to Bacillus endophyticus with 100% sequence similarity.
The selection of siderophore producing bacteria was studied. It was found that bacteria ISP2RS6 and S213 produced hydroxamate and catecholate siderophore in King s' B medium at 295.02, 275.19, 107.42 and 61.11 ?mol/L, respectively. It also found that bacteria S59 and S65 could produced hydroxamate and catecholate siderophore at 277.55 and 67.40 ?mol/L, respectively. Four selected bacteria produced maximum siderophore content at 48 h. The identification of selected siderophore producing bacteria found that ISP2RS6 and S59 were Gram positive and bacteria S65 and S213 were Gram negative bacteria. The 16S rRNA gene sequencing of ISP2RS6, S59, S65 and S213 were identified as Bacillus pumilus, Bacillus safensis, Alcaligenes aquatilis and Salmonella enterica at 100, 100, 98 and 100 % similarity, respectively.
Bacillus endophyticus S70 (ACC deaminase producing bacteria) and Bacillus pumilus ISP2RS6 (siderophore producing bacteria) and both of them were phosphate solubilizing bacteria should be applied to organic rice farming system for reduction the effect of biotic and abiotic stress including stress under climate change. Moreover, their bioactive compounds biotechnological applications should be