This quantitative research article aimed to 1) Examine the levels of organizational culture, human resource practices, work-life balance, and intention to stay of Generation Z personnel in Ministry of Public Health agencies, Chiang Mai Province; and 2) Analyze the influence of these factors on intention to stay. The sample consisted of 300 personnel born between 1997 and 2007 who were working in regional hospitals, general hospitals, district public health offices, and sub-district health promoting hospitals in Chiang Mai Province, covering civil servants, Ministry of Public Health employees, wage employees, and service contractors. They were selected through purposive sampling, and data were collected using an online questionnaire and analyzed using descriptive statistics and multiple regression analysis. The findings revealed that 1) Generation Z personnel rated all four variables at a high level: organizational culture (??? = 4.18, S.D. = 0.49), human resource practices (??? = 4.07, S.D. = 0.54), intention to stay (??? = 4.03, S.D. = 0.71), and work-life balance (??? = 3.74, S.D. = 0.79); and 2) Organizational culture, human resource practices, and work-life balance had statistically significant positive influences on intention to stay at p < 0.01 (R = 0.718, R? = 0.516, Adj. R? = 0.511), with human resource practices exerting the greatest influence (? = 0.407), followed by work-life balance (? = 0.259) and organizational culture (? = 0.163). Public health agencies should therefore review compensation and benefits, introduce flexible shift scheduling, reduce overtime, and design clear career paths for personnel of all employment types.