The research aims to investigate the flora and fauna diversity of Khao Plu Cave Hot Spring and to propose guidelines for the impact surveillance of Khao Plu Cave Hot Spring, Lamae district, Chumphon province, under the concept of sustainable tourism. Wildlife sighting surveys and plant species surveys were used to collect darra. According to the findings, plant species found at Khao Plu Cave Hot Spring indicate two types of forest: evergreen and swamp. There were 51 families, 116 species, 56 species of woody plants, 19 species of shrubs, 23 species of vines, 8 species of herbaceous plants, 7 types of palms and 3 types of ferns. There were several notable plants in the evergreen forest, including Hopea odorata Roxb., Parkia timariana Merr., Diospyros pubicalyx Bakh. Besides, there were also many prominent plants in the swamp forest, such as Pandanus kaida Kurz., Salacca wallichiana C.Msrt., Vitex pinnata Linn., Eugenia claviflora Roxb., and Caryota bacsonensis Magalon. There were 22 bat families and 39 species of bats, monkeys, squirrels, and birds, all of which were endemic. There were 34 bird species in urban areas, 36 in agricultural areas, and 33 in natural forests. However, out of 39 species, 7 were found the most frequently, 13 were found frequently, 12 were found moderately, and 7 were found rarely. As a result, before developing recreational and tourism facilities in the area, Khao Plu Cave Hot Springs should investigate the potential impacts on resources, particularly wildlife and plant species habitats.